Source Operations

Source operations create the initial Pools from which all downstream Pools are constructed. They do not require an existing pool as input. Sources may represent user-specified sequences, DNA sequences generated using IUPAC ambiguity codes or position weight matrices, random k-mers, constrained barcodes, and more.

from_seq	Create a pool from a single sequence string, optionally with region tags.
from_seqs	Create a pool that draws uniformly from a list of sequences.
from_fasta	Load sequences from a FASTA file.
from_iupac	Enumerate all sequences consistent with an IUPAC ambiguity string.
from_motif	Sample sequences from a position-probability matrix.
get_kmers	Enumerate every k-mer of a given length over a specified alphabet.
get_barcodes	Generate DNA barcodes satisfying distance, GC, and homopolymer constraints.