Tutorials

Step-by-step guides that walk through complete library design workflows. Each tutorial builds a realistic library from scratch, explaining design decisions along the way.

• Deep Mutational Scanning: Protein GB1 — build a deep mutational scanning library for protein GB1, combining single and pairwise amino acid substitutions, random higher-order mutants, and wild-type replicates.

• MPRA Library for Regulatory Grammar — design an MPRA library that places three transcription factor binding sites at random positions within a regulatory element and pairs each variant with a unique barcode.