rc

Return the reverse complement of a sequence or a named region. By default the entire sequence is reverse-complemented and region tags are stripped; pass region= to restrict the operation to a single tagged segment.

import poolparty as pp
pp.init()

Parameters

Parameter	Type	Default	Description
`pool`	`Pool \| str`	(required)	Pool or sequence string to reverse complement.
`region`	`str \| list \| None`	`None`	Named region tag, `[start, stop]` interval, or `None` for the full sequence.
`remove_tags`	`bool \| None`	`None`	If `True` when `region` is a marker name, strip region tags from the output; `None` uses the operation defaults.
`iter_order`	`int \| float \| None`	`None`	Dimension-name ordering for downstream multi-pool iteration.
`prefix`	`str \| None`	`None`	Prefix for sequence names in the resulting pool.
`style`	`str \| None`	`None`	Display style passed to `stylize()` on the result.

Note

Only the most commonly used parameters are shown above. For the full parameter list, see rc() in the API Reference.

Examples

Reverse complement a simple sequence

Take the reverse complement of the 4-mer ATCG; the result is CGAT.

import poolparty as pp
pp.init()

wt = pp.from_seq("ATCG")
r  = pp.rc(wt)
r.print_library()

r: seq_length=4, num_states=1 CGAT

Reverse complement a longer tagged sequence

Reverse-complement the full sequence AAAA<cre>ATCGATCG</cre>TTTT (tags are stripped before the operation and not carried into the output).

import poolparty as pp
pp.init()

wt = pp.from_seq("AAAA<cre>ATCGATCG</cre>TTTT")
r  = pp.rc(wt)
r.print_library()

r: seq_length=16, num_states=1 AAAACGATCGATTTTT

Reverse complement only a named region

Pass region= to reverse-complement only the content of the cre segment; flanking sequences are returned unchanged.

import poolparty as pp
pp.init()

wt = pp.from_seq("AAAA<cre>ATCGATCG</cre>TTTT")
r  = pp.rc(wt, region="cre")
r.print_library()

r: seq_length=8, num_states=1 AAAA<cre>CGATCGAT</cre>TTTT

See rc().