Mutagenesis Operations

Mutagenesis operations introduce random variation into sequences through point mutations, base shuffling, recombination, or orientation flipping. These operations are stochastic by default and produce a different draw each time, though most support mode='sequential' for exhaustive enumeration of all possible variants.

import poolparty as pp
pp.init()

mutagenize	Introduce random point mutations at a specified rate or count.
shuffle_seq	Randomly permute the bases of a sequence or a tagged region.
recombine	Produce chimeric sequences by recombining with source sequences at random breakpoints.
flip	Produce forward and reverse-complement variants as distinct pool states.